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Functionalized surfaces – how phases become instrAction phases

The innovative instrAction gels are produced through the modification of the surfaces of different carrier materials. By applying a polymer layer, a reticulation develops into which a number of different ligand combinations can be inserted.

The instrAction technology guaranties a regulation of the ligand density (in mm/ml) which is easy to control. Conventional chromatographic approaches make use of a separation based on the irregular distribution of the target molecule and the minor components to the mobile and stationary phase. This is due to the varying degree of a monomodal interaction (e.g. an ionic interaction) or solubility. In conventional chromatography, only one possible interaction of target molecule and chromatographic phase is used for a separation. However, instrAction phases are based on a multimodal approach which means on simultaneous, diverse and non-covalent interactions. Through the choice of ligands, an ideal combination of e.g. ionic, lipophilic, hydrophilic and hydrogen bridge bonds, as well as π-interactions of a single instrAction phase, with the desired target molecule is created. InstrAction phases’ affinity to the target molecule is achieved by its specific combination, distribution and density of its ligands.

The development of specific polishing products for pharmaceutical active substances is a particularly impressing example of this technology: here, molecules that were strongly related concerning their structure, had been completely separated and extracted without any losses. Even minimal structural differences like the deamidation of a peptide’s single amino acids can have an immense separation efficiency on instrAction phases.

What is the secret behind the extreme durability of the instrAction phases that can easily be sanitized with all common methods? It is the usage of internal carriers and chemically resistant ligands, as well as the covalent binding by means of stable chemical bonds. There are no limits in terms of applicable solvents, modifiers and separation conditions.